| Autor: |
Gruijter, Johanna M., Lieshout, Lisette, Gasser, Robin B., Verweij, Jaco J., Brienen, Eric A. T., Ziem, Juventus B., Yelifari, Lawrence, Polderman, Anton M. |
| Předmět: |
|
| Zdroj: |
Tropical Medicine & International Health; Jun2005, Vol. 10 Issue 6, p574-580, 7p |
| Abstrakt: |
We evaluated a two-step semi-nested polymerase chain reaction (PCR)-based approach for the specific detection ofAncylostoma duodenaleDNA in human faeces. The test was used to determine to what extent this species of hookworm is present in the regions of Bolgatanga and Garu of northern Ghana. Initially, the sensitivity and specificity of the PCR were tested using a range of well-defined control samples. Subsequently, a total of 378 human faecal DNA samples from Bolgatanga and Garu were subjected to the PCR. The results were compared with those obtained using a previously established PCR for the specific detection ofNecator americanusDNA in human faeces. Infection withA. duodenalewas recorded in 74 (19.6%) samples andN. americanusin 278 (73.5%), of which 64 (16.9%), represented co-infections with both species. WhileA. duodenalewas predominantly detected in the samples from Bolgatanga, infections in Garu related almost exclusively toN. americanus. The results showed that the present PCR approach is a valuable complementary tool for the diagnosis ofA. duodenaleinfection in humans in Ghana, having implications for epidemiological studies and for the monitoring of the success of control programmes in regions in Africa. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
Plný text