| Abstrakt: |
The very hazardous and persistent aquatic pollutants known as polychlorinated biphenyls (PCBs) are known to bioaccumulate in a range of marine mammals. Human and animal studies have shown that exposure to PCBs affects the male reproductive system. This study was conducted to investigate the therapeutic effects of D-ribose-L-cysteine (DRLC) on PCB-mediated ATPase disruption of the neuroendocrine transmembrane ion pump and peroxynitrite formation in rat models. After the first 15 days of PCB treatment, DRLC (50 mg/kg) was administered orally for 15 days, beginning on day 16 and continuing through day 30. PCB (2 mg/kg) was administered for 30 days. All tests were performed in accordance with accepted procedures. Kisspeptin (kissp), gonadotropin-releasing hormone alkaline phosphatase (ALP), lactate dehydrogenase (LDH), glutathione peroxidase (GSH-Px), and total antioxidant capacity (TAOC) were all analyzed in blood on day 31. The testes were also removed and sent for testosterone, testicular antioxidant enzymes (L-C and PrDx-4), androgenic enzymes (3ß-HSD and 17ß-HSD), sulfhydryl content, and transmembrane ion pump (Na+, K+, Ca2+, Mg2+, and H+ ions) The results of the study showed that Kisspeptin, GnRH, Testosterone, L-C, PrDx-4, 3-HSD, 17-HSD, HOST, Na+, K+, Ca2+, Mg2+, and H+-ATPase levels were significantly increased by DRLC. In addition, DRLC therapy dramatically reduced levels of ONOO-, H2O2, 8-OHdG, ALP, and LDH, while increasing TAOC to restore GSH-Px levels. The results of the present study suggest that DRLC is a potentially effective therapeutic approach to reduce PCB-induced neuroendocrine transmembrane ion pump disruption in an experimental rat model. The primary mechanism underlying this effect may be its antioxidant activities against the formation of peroxynitrite and elevated levels of hydrogen peroxide. [ABSTRACT FROM AUTHOR] |
