| Abstrakt: |
As the world's population increases, so does the need for livestock-based nutrition. In addition, the livestock sector becomes more important as it contributes to the economy. However, sepsis has high morbidity and mortality rate in newborn calves and can cause severe economic losses. Therefore, new biomarkers to distinguish sepsis from other diseases are urgently needed in veterinary medicine. This study, for the first time, examined the gene expression levels of members of the necroptosis pathway, such as receptor-interacting serine/threonine protein kinase 1 (RIPK1), RIPK-3, and one of the NF-κB activating proteins, RIPK-2, in septic calves. We examined the mRNA levels of RIPK1, RIPK3, and RIPK2 using qPCR in 10 healthy Holstein calves and 20 Holstein calves with sepsis due to suffering from enteritis infection between 1-20 days of age. The hematologic parameters, including leukocytes, erythrocytes, hemoglobin, hematocrit, and platelets, were also evaluated in the calves included in this study. The results showed that calves with sepsis had prominently lower mRNA levels of RIPK1 and RIPK3 than those in healthy calves. Besides, RIPK2 mRNA expression was absent in healthy calves and calves sepsis. In veterinary medicine, decreased RIPK1 and RIPK3 mRNA levels might be biomarkers to diagnose sepsis in calves. [ABSTRACT FROM AUTHOR] |
