| Abstrakt: |
In-depth identification of crop germplasm resources is the premise and basis for breeding and utilization. The Crop Genebank of Yunnan Province has preserved abundant wheat germplasm resources in Yunnan, but the resistance characteristics of stripe rust are still unclear. In this study, 260 Yunnan landrace wheat germplasm resources preserved in the Crop Genebank were determined by carrying out the identification of stripe rust resistance at adult plant stage in the field and the genotype analysis of three known resistance genes Yr5, Yr10 and Yr15, to clear the resistance level of stripe rust and the distribution of three known resistance genes Yr5, Yr10 and Yr15, which provided a theoretical reference for the screening of excellent resistance resources and the exploration and utilization of resistance genes. The results showed that 38 of 260 Yunnan landrace wheat germplasm resources showed immune and near immune resistance to stripe rust at adult stage in Songming experimental site in 2022, accounting for 14.62 %. There were 93 high resistance materials, accounting for 35.77 %, 46 medium resistance materials, accounting for 17.69 %, and 83 medium and high susceptible materials, accounting for 31.92 %. At the same time, using capillary electrophoresis detection technology, there were 11 materials carrying Yr10 resistance gene in 260 Yunnan landrace wheat germplasm resources, accounting for 4.23 %, and 0 materials carrying Yr5 and Yr15 resistance genes. In summary, 260 Yunnan landrace wheat resources are rich in stripe rust resistance materials. The proportion of immune, near immune and high resistance materials are 50.39 %, and resistance genes Yr5 and Yr15 are undetected. It is speculated that the above materials are likely to carry unknown new resistance genes. [ABSTRACT FROM AUTHOR] |
