| Autor: |
Uto, Sahrani, Arfah, Rugaiyah, Dali, Seniwati, Baharuddin, Maswati |
| Předmět: |
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| Zdroj: |
Molekul: Jurnal Ilmiah Kimia Molekul; Jul2023, Vol. 18 Issue 2, p227-235, 9p |
| Abstrakt: |
Oryctes rhinoceros L. has the ability to produce enzymes since its digestive system contains 65% usable symbiotic bacteria. The aims of the research were to isolate and phenotypic identify the amylolytic bacteria that produce amylase enzymes from horn beetle larvae. The methods involve using isolated and screened bacteria. The morphology and biochemical of the isolates were described. The detected isolate's genomic DNA was isolated and 16S rRNA sequencing was used to examine it. The results of screening and bacterial isolation obtained five isolates that produced amylase. Five selected bacterial isolates had amylolytic index ranging from 0.135-1.370 mm at 37 oC. The five bacterial isolates were rod-shaped gram-negative bacteria. Based on the 16S rRNA gene sequence and phylogenetic tree analysis showed that EA1 is closely related to Ochrobactrum sp with a similarity level of 97.71% and EA2 is related to Pseudomonas mendocina with a similarity level of 98.52%. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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