| Autor: |
Hermans, Leen, Denaeghel, Sofie, Jansens, Robert J. J., De Pelsmaeker, Steffi, Van Nieuwerburgh, Filip, Deforce, Dieter, Hegewisch-Solloa, Everardo, Mace, Emily M., Cox, Eric, Devriendt, Bert, Favoreel, Herman W. |
| Předmět: |
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| Zdroj: |
Frontiers in Immunology; 2023, p1-13, 13p |
| Abstrakt: |
Liver-resident NK (lrNK) cells have been studied in humans as well as in mice. Unfortunately, important differences have been observed between murine and human lrNK cells, complicating the extrapolation of data obtained in mice to man. We previously described two NK cell subsets in the porcine liver: A CD8ahigh subset, with a phenotype much like conventional CD8ahigh NK cells found in the peripheral blood, and a specific liver-resident CD8adim subset which phenotypically strongly resembles human lrNK cells. These data suggest that the pig might be an attractive model for studying lrNK cell biology. In the current study, we used RNA-seq to compare the transcriptome of three porcine NK cell populations: Conventional CD8ahigh NK cells from peripheral blood (cNK cells), CD8ahigh NK cells isolated from the liver, and the liver-specific CD8adim NK cells. We found that highly expressed transcripts in the CD8adim lrNK cell population mainly include genes associated with the (adaptive) immune response, whereas transcripts associated with cell migration and extravasation are much less expressed in this subset compared to cNK cells. Overall, our data indicate that CD8adim lrNK cells show an immature and anti-inflammatory phenotype. Interestingly, we also observed that the CD8ahigh NK cell population that is present in the liver appears to represent a population with an intermediate phenotype. Indeed, while the transcriptome of these cells largely overlaps with that of cNK cells, they also express transcripts associated with liver residency, in particular CXCR6. The current, in-depth characterization of the transcriptome of porcine liver NK cell populations provides a basis to use the pig model for research into liver-resident NK cells. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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