| Autor: |
Ullah, Muhammad Asad, Gul, Faiza Zareen, Zaman, Gouhar, Iqbal, Junaid, Drouet, Samantha, Tungmunnithum, Duangjai, Hano, Christophe, Abbasi, Bilal Haider |
| Zdroj: |
Acta Physiologiae Plantarum; Sep2023, Vol. 45 Issue 9, p1-11, 11p |
| Abstrakt: |
Ajuga integrifolia Buch. Ham. ex D.Don (Family: Lamiaceae) is a wild crafted perennial medicinal herb. Ajuga integrifolia rapid micro-propagation was induced to get disease-free plantlets and high yield agitated micro-shoot cultures by in vitro shoot culture. Micro-shoots were supplemented with Chitosan (CHT) and Melatonin (MEL) in a dose-dependent manner to elicit the secondary metabolites and biomass accumulation. Biomass accumulation was increased [dry weight (DW 16.8 g/L)] in response to CHT (10 mg/L) comparative to control and MEL. A total of 11 bioactive compounds were quantified by HPLC. Results illustrated that MEL (5 mg/L) was optimum for accumulation of majority of bioactive compounds. However, optimum production of aucubin, harpagoside, catalpol, and 8-O-acetyl harpagoside was noted at MEL (3 mg/L) concentration, whereas maximum harpagide production showed by CHT (150 mg/L) treated cultures. Anti-inflammatory and antioxidant activities were highest by MEL (3 mg/L) and MEL (5 mg/L), respectively, correlated with phytochemical biosynthesis. According to the results, Ajuga integrifolia agitated micro-shoot cultures treated with melatonin might be an efficient bio-factory of pharmaceutically active compounds especially chlorogenic acid, rosmarinic acid, apigenin, and quercetin. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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