| Abstrakt: |
To survey the estrogenic activity of the organic extracts from particulate matter of urban ambient outdoor air, samples were collected on glass fiber filters using a high-volume air sampler on the rooftop of our institute for 6 months (six filters/month). After extracting the organic materials and separating them into three fractions, i.e., acidic, neutral, and basic, we applied a cell-growth assay using MCF-7 human breast cancer cells to the original extract and the extracts of the fractions. Only the extract in the acidic fraction showed cell proliferation activity in a dose-response manner. To survey the chemical(s) responsible for the activity, a gas chromatography/mass spectrometry (GC/MS) analysis was conducted after silylating the extract. The presence of bisphenol A (BPA) was confirmed, because the retention times and the MS fragment patterns between the silylated derivative of a component in the sample and that of BPA itself were the same. By using a GC/MS-SIM (selective ion monitoring) technique, the average value was found to be 0.51 ng/m3 of air (range: 0.02 ~ 1.92 ng/m3 of air). The trend of the residual levels in air particulates showed seasonal variation, increasing from autumn to winter and decreasing from winter to spring. The only exception was that the value in January was lower than those in December and February. Considering the content of BPA in the extract of the acidic fraction and the strength of the activities with the extract and BPA itself, the estrogenic activity due to BPA in the fraction seemed to decrease. In spite of this decline, the possibility remains that the estrogenic activity mainly originated from BPA. [ABSTRACT FROM AUTHOR] |
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