| Autor: |
Barton, Penny, Robinson, Nick, Middleton, Sonya, O'Brien, Amanda, Clarke, John, Dominguez, Maria, Gillgan, Steve, Selmes, John, Rhodes, Shelley |
| Předmět: |
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| Zdroj: |
Veterinary Sciences; Aug2023, Vol. 10 Issue 8, p489, 15p |
| Abstrakt: |
Simple Summary: This study describes the evaluation of four antibody tests (three ELISA tests and one lateral flow) for the detection of Mycobacterium bovis infection in deer and pigs. Test sensitivity and specificity were derived for each test with associated test cut-offs. There was a high level of test agreement between the tests. High test specificity was achieved, with a high to moderate test sensitivity, depending on whether or not a prior skin test had been performed, respectively. These data allow for the confident use of antibody tests for pigs and deer in GB, where previously none were available. This study addressed the need in Great Britain for supplementary blood tests for deer and pig herds under movement restrictions due to confirmed Mycobacterium bovis infection—to enhance the overall sensitivity and reliability of tuberculosis (TB) testing and contribute to an exit strategy for these herds. We evaluated four antibody tests (lateral flow DPP VetTB Assay for Cervids, M. bovis IDEXX ELISA, Enferplex Cervid and Porcine antibody tests and an in-house comparative PPD ELISA) using serum samples from defined cohorts of TB-infected and TB-free deer and pigs. TB-infected deer included two separate cohorts; farmed deer that had received a tuberculin skin test less than 30 days prior, and park deer that had received no prior skin test. In this way, we were able to assess the effect of the skin test anamnestic boost upon antibody test sensitivity. We tested a total of 402 TB-free pigs and 416 TB-free deer, 77 infected farmed deer and 105 infected park deer, and 29 infected pigs (including 2 wild boar). For deer, we found an equivalent high performance of all four tests: specificity range 98.8–99.5% and sensitivity range 76.6–85.7% for skin test-boosted infected deer, and 51.4–58.1% for non-boosted infected deer. These data suggest an overall approximate 25% increase in test sensitivity for infected deer following a skin test boost. For pigs, the tests again had equivalent high specificity of 99–99.5% and a sensitivity range of 62.1–86.2%, with substantial agreement for three of the four tests. Retrospective application of the ELISA tests to individual culled park deer and wild boar that showed no obvious evidence of TB at larder inspection identified a significant seropositivity within wild boar suggestive of low-level M. bovis infection that would otherwise not have been detected. Overall this investigation provided a robust evaluation of four antibody tests, which is essential to generate confidence in test performance before a wider deployment within TB control measures can be considered. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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