| Autor: |
Tabuchi, Mayumi, Sugiyama, Kenjiro, Ishiyama, Keiki, Inoue, Eri, Sato, Tadashi, Takahashi, Hideki, Yamaya, Tomoyuki |
| Předmět: |
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| Zdroj: |
Plant Journal; Jun2005, Vol. 42 Issue 5, p641-651, 11p |
| Abstrakt: |
Rice (Oryza sativaL.) plants possess three homologous but distinct genes for cytosolic glutamine synthetase (GS1): these areOsGS1;1,OsGS1;2, andOsGS1;3.OsGS1;1was expressed in all organs tested with higher expression in leaf blades, whileOsGS1;2, andOsGS1;3were expressed mainly in roots and spikelets, respectively. We characterized knockout mutants caused by insertion of endogenous retrotransposonTos17into the exon-8 (lines ND8037 and ND9801) or the exon-10 (line NC2327) ofOsGS1;1. Mendelian segregation occurred in each progeny. Homozygously inserted mutants showed severe retardation in growth rate and grain filling when grown at normal nitrogen concentrations. Abnormal mRNA for GS1;1 was transcribed, and the GS1 protein and its activity in the leaf blades were barely detectable in these mutants. The glutamine pool in the roots and leaf blades of the mutants was lower than that of the wild type. Re-introduction ofOsGS1;1cDNA under the control of its own promoter into the mutants successfully complemented these phenotypes. Progeny whereTos17was heterozygously inserted or deleted during segregation showed normal phenotypes. The results indicate that GS1;1 is important for normal growth and grain filling in rice; GS1;2 and GS1;3 were not able to compensate for GS1;1 function. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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