| Autor: |
Takase, Tomomasa, Yanagawa, Yuki, Komatsu, Setsuko, Nakagawa, Hiroki, Hashimoto, Junji |
| Předmět: |
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| Zdroj: |
Journal of Plant Research; Dec2003, Vol. 116 Issue 6, p469-475, 7p, 4 Diagrams, 1 Chart, 2 Graphs |
| Abstrakt: |
To understand the cell cycle process in plants, we searched for proteins that quantitatively change during the cell cycle in suspension-cultured rice (Oryza sativa L.) cells. The proteins were analyzed by a two-dimensional polyacrylamide gel electrophoresis image-analysis system. We detected 11 proteins that quantitatively changed during the cell cycle, among which β-tubulins and a calreticulin-like protein were identified. The amounts of β-tubulin proteins were low in the M phase and high in the G1 phase. In contrast, mRNAs for two of the three types of β-tubulin were high in the M phase of the cell cycle. The addition of protease inhibitors MG132 or E64d to the cells decreased the β-tubulin proteins during 24 h, suggesting that β-tubulin proteins are degraded in vivo by proteases other than those whose activities are inhibited by MG132 or E64d. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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