| Autor: |
Roth, David M., Drumm, Jeffrey D., Bhargava, Valmik, Swaney, James S., Gao, Mei Hua, Hammond, Kirk |
| Předmět: |
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| Zdroj: |
Basic Research in Cardiology; Nov2003, Vol. 98 Issue 6, p380-387, 8p |
| Abstrakt: |
Mice with cardiac-directed overexpression of ACVI show increased cardiac responsiveness to β-adrenergic receptor stimulation but regulation of heart rate is unknown. Telemetry was used to test the hypothesis that mice overexpressing cardiac adenylyl cyclase type VI (ACVI) would have normal heart rate regulation. Mice overexpressing cardiac ACVI were generated using the αMHC promoter and studied 10 days after implantation of telemetry devices. Cardiac transgene ACVI presence and expression was verified using PCR, RT-PCR and immunoblotting. Ambulatory heart rates were assessed using time and frequency domain analysis over two 24 hour light-dark cycles. Heart rates then were assessed following pharmacological blockade. Time domain analyses showed ambulatory heart rates were unchanged (ACVI: 597 ± 15 (SEM) bpm, Control: 595 ± 12 bpm; p = 0.92). Circadian heart rate variability was preserved and not different from control mice (ANOVA, p = 0.52). Frequency domain analysis of heart rate variability also was unchanged. No difference in heart rate response to pharmacological autonomic blockade was found (intrinsic heart rate: ACVI 622 ± 17 bpm, control 616 ± 16 bpm, p = 0.79). In conclusion, mice overexpressing cardiac ACVI have normal conscious ambulatory heart rates and normal heart rate variability. Overexpression of cardiac ACVI does not result in altered heart rate regulation in contrast to cardiac overexpression of other elements of the β-adrenergic signaling pathway. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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