| Autor: |
Perez de Souza, Leonardo, Bitocchi, Elena, Papa, Roberto, Tohge, Takayuki, Fernie, Alisdair R. |
| Předmět: |
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| Zdroj: |
Plant Journal; Aug2023, Vol. 115 Issue 4, p1021-1036, 16p |
| Abstrakt: |
SUMMARY: The process of crop domestication leads to a dramatic reduction in the gene expression associated with metabolic diversity. Genes involved in specialized metabolism appear to be particularly affected. Although there is ample evidence of these effects at the genetic level, a reduction in diversity at the metabolite level has been taken for granted despite having never been adequately accessed and quantified. Here we leveraged the high coverage of ultra high performance liquid chromatography‐high‐resolution mass spectrometry based metabolomics to investigate the metabolic diversity in the common bean (Phaseolus vulgaris). Information theory highlights a shift towards lower metabolic diversity and specialization when comparing wild and domesticated bean accessions. Moreover, molecular networking approaches facilitated a broader metabolite annotation than achieved to date, and its integration with gene expression data uncovers a metabolic shift from specialized metabolism towards central metabolism upon domestication of this crop. Significance Statement: Using untargeted metabolomics, molecular networking, and information theory, we quantified and characterized, at the metabolite level, the reduction in metabolic diversity decurrent of common bean domestication. Our results suggest a shift of carbon flux from specialized metabolites towards central metabolism. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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