| Abstrakt: |
Sea daffodil (Pancratium maritimum L.), which belongs to the Amaryllidaceae family, grows in sandy areas along coastal regions and its population is at risk due to the exploitation of its natural resources for medicinal purposes, tourism, and urbanization. Micropropagation is employed to conserve and sustain endangered plant species like P. maritimum. In this investigation, different types of explants (leaf, root, and mature zygotic embryos) from P. maritimum were cultured on Murashige-Skoog (MS) medium supplemented with 2,4-Dichlorophenoxyacetic acid (2,4-D) (1, 2, 4 mg l−1) and 6-Benzyladenine (BA) (0 and 1 mg l−1) for callus induction. The callus formation rate, callus growth rate, embryogenic callus rate, and callus type were evaluated. The induced calli were further tested for shoot formation on MS medium supplemented with 2 mg l−1 BA and 0.2 and 0.5 mg l−1 2,4-D. The results showed that the highest callus induction was achieved using zygotic embryo explants and the medium containing both 2,4-D and BA. Successful shoot formation from callus was determined using both MS media supplemented with 2 mg l−1 BA and 0.5 or 0.2 mg l−1 2,4-D, with a success rate of 90%. To induce in vitro bulb formation of P. maritimum plantlets, MS medium containing varying concentrations of sucrose (20, 40, and 80 g l−1), BA (0, 1, and 2 mg l−1), and 2,4-D (0, 0.1, and 0.2 mg l−1) was utilized. The bulb formation rate of P. maritimum was successful in all growth media, ranging from 60 to 82%. The diameter of the bulb was found to increase with higher sucrose concentration (80 g l−1) in the growth medium. The impact of plant growth regulators on bulb weight was more pronounced in nutrient medium containing low sucrose concentration (20 g l−1). Efficient protocols for embryogenic callus induction, organogenesis, regeneration, bulb formation, and acclimatization were developed for P. maritimum, providing valuable insights for future studies. Key message: Pancratium maritimum is an endangered plant with medicinal and decorative value. We have successfully developed a comprehensive protocol for its micropropagation, including callus induction, regeneration, bulb formation, and acclimatization. [ABSTRACT FROM AUTHOR] |
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