| Autor: |
Cuong, Do Manh, Mai, Nguyen Thi Nhu, Tung, Hoang Thanh, Khai, Hoang Dac, Luan, Vu Quoc, Phong, Truong Hoai, Dinh, Tran Minh, Vinh, Nguyen Quang, Dung, Doan Manh, Dung, Nguyen Anh, Uyen, Huynh To, Huyen, Nguyen Thi, Oanh, Do Tu, Nhut, Duong Tan |
| Zdroj: |
Plant Cell, Tissue & Organ Culture; Sep2023, Vol. 154 Issue 3, p605-618, 14p |
| Abstrakt: |
In this study, the positive effect of AgNPs solution at the concentration of 2 mg/L on the in vitro shoot multiplication of Chrysanthemum was recored; this experiment was carried out to improve shoot quality and produce a large number of material sources for our experiments. Phytohormones are organic substances synthesized during plant metabolism. They have an obvious physiological effect on plant growth at very low concentrations. Phytohormones are found not only in higher plants but also in algae, bacteria and fungi related to plants. Previous studies have focused on determining which microorganisms can biosynthesize phytohormones and their effects on plant growth and development and stress tolerance under ex vitro conditions; however, few studies evaluate the effects of these compounds on in vitro plant growth. In this study, the effect of a phytohormone mixture (zeatin, IAA and GA3) produced by Pseudomonas otitidis YWY-9.1 on the growth and development of Chrysanthemum plantlets was determined for the first time. After 30 days of culture, physiological parameters such as plantlet height (6.17 cm), number of stem nodes (4.70 stem nodes/plantlet), number of leaves (10.33 leaves/plantlet), chlorophyll content (27.00 nmol/cm2), number of roots (4.67 roots/plantlet), root length (6.17 cm), fresh weight (6.17 mg) and dry weight (0.077 mg) of Chrysanthemum plants cultured on medium supplemented with 10 mL/L of phytohormones sterilized by filter was superior to that of the other treatments and the control. Besides, the content of endogenous hormones and antioxidant enzymes in Chrysanthemum plants grown on this medium is also more optimal. Furthermore, the plantlets derived from the medium containing phytohormones had better acclimatization and growth under ex vitro conditions than the control. However, when adding phytohormones at high concentration (15 mL/L), the growth and development parameters of Chrysanthemum was inhibited, reducing the quality of plantlets. Moreover, when adding phytohormones derived from microorganisms to the culture medium, a syringe filter should be used, avoiding sterilization at high temperature so as not to affect the phytohormone's activity. Key message: Supplementation of phytohormones produced by Pseudomonas otitidis YWY-9.1 in culture medium positively effects on growth and development of Chrysanthemum plantlets. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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