| Abstrakt: |
Curcumin is a natural polyphenol compound found in turmeric rhizomes, which is frequently used in the food and pharmaceutical industries. Turmeric is widely distributed in East and South East Asia. Turmeric is currently consumed heavily in Asian countries in the of 200-1000 mg/day or 160-440 g/person/year due to potential therapeutic applications resulting in the extraction of important bioactive compounds such as curcumin has attracted much attention. However, curcumin's limitations greatly affect extraction efficiency: not stable to visible light, degrades at high temperatures, and poor water solubility, and decomposes very quickly in aqueous solutions at alkaline pH, resulting in the choice of extraction method important to maximum extraction yield. Curcumin was separated successfully from turmeric rhizomes using a new and easy-to-industrialized extraction method called "Aqueous two-phase system (ATPS)". ATPS consisting of ammonium sulfate/ethanol/water was used in this curcumin extraction study, with the extracted curcumin samples was confirmed of curcumin was carried out by UV-vis spectroscopy. An experimental design and response surface method (RSM) were utilized to determine the optimal values of ethanol concentration, ammonium sulfate concentration, solvent to solid ratio, and extraction temperature to attain maximum extraction yield. The maximum yield for curcumin (99%) from the experiment was consistent with the prediction by the regression model, showing good agreement. In addition, equilibrium and kinetic extraction characteristics were studied, and found that the second-order kinetics correlates well with the experimental data. [ABSTRACT FROM AUTHOR] |
