| Abstrakt: |
Corn represents one of the most significant available biomass sources. Here, cornmeal, a product made of grounded corn, was hydrolyzed by three different enzymatic cocktails: (a) extract rich in Aspergillus brasiliensis amylases (AAB), (b) rich extract in Aspergillus tamari Kita xylanases (XAT), and (c) rich commercial extract in Trichoderma reesei cellulases (CTR). The mixture design results, containing the three different cocktails, showed that AAB action on cornmeal does not require XAT or CTR supplementation, being considered the most efficient. The optimum pH and temperature for amylolytic activity were investigated, and the pH 3.75–4.6 and 60–70 °C ranges were the best conditions for amylase activity. The cornmeal hydrolysis optimization showed that 5% substrate and 48 h of incubation with AAB were the best parameters for cornmeal conversion into simple sugars. The central composite design enabled an adequate and reliable measurement of cornmeal hydrolysis, pH 4.0–4.5 and 40–45 °C ranges resulted in 32.4–37.8 g/L of reducing sugar release. [ABSTRACT FROM AUTHOR] |
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