| Abstrakt: |
Superoxide dismutase (SOD) is extremely important antioxidant enzyme and plays essential roles in plant growth, development, and response to different abiotic stresses. The SODs genes family has been characterized in several plant species; however, there is no report on identifying these genes in Coffea spp. In this study, the objective was to identify and analyze in silico the SODs genes using the three genomes of Coffea spp. We used various bioinformatics tools to perform physicochemical analyses of proteins, gene structure, chromosome mapping, conserved domain, cis-acting elements, syntenic and phylogenetic analyses, and miRNA targets. A total of 32 SODs genes were identified in the genomes of Coffea spp. (9—C. canephora; 13—C. arabica L.; 9—C. eugenioides), and phylogenetically classified into 3 subgroups, copper zinc (Cu/Zn-SOD/CSD), manganese (Mn-SOD/MSD), and iron (Fe-SOD/FSD), respectively. Physicochemical analyses of the coffee SODs proteins revealed that the length, molecular weight, and pI values varied between species from 105 (CcCSD4) to 317 (CeCSD7) amino acids, 12.00 (CeCSD4) to 34.59 (CeFSD1) kDa, and 5.37 (CeFSD1) at 9.35 pI (CeCSD4). The intron numbers of SODs genes ranged from 1 to 8. Chromosomal mapping revealed an uneven distribution of SODs genes in the chromosomes of the respective coffee species. In addition, we identified eight stress-responsive cis-elements in the promoters of coffee SODs genes. In addition, we identified a total of 13, 12, and 11 putative miRNA families, which can target genes encoding SODs in the 3 coffee species. This study will serve as a biotechnological basis for the characterization of SODs genes involved in regulating gene expression under adverse conditions. This allows the development of biotechnological strategies to ensure greater sustainability of coffee production by establishing ways to reduce damage caused by stressful conditions in coffee production. [ABSTRACT FROM AUTHOR] |
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