| Autor: |
Aertsen, Abram, Masschalck, Barbara, Wuytack, Elke Y., Michiels, Chris W. |
| Předmět: |
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| Zdroj: |
Extremophiles; Dec2003, Vol. 7 Issue 6, p499-504, 6p |
| Abstrakt: |
Sodium concentrations as low as 2 mM exerted a significant protective effect on the high-pressure inactivation (160–210 MPa) of Rhodotorula rubra at pH 6.5, but not on two other yeasts tested (Shizosaccharomyces pombe and Saccharomyces cerevisiae). A piezoprotective effect of similar magnitude was observed with Li+ (2 and 10 mM), and at elevated pH (8.0–9.0), but no effect was seen with K+, Ca2+, Mg2+, Mn2+, or NH4+. Intracellular Na+ levels in cells exposed to low concentrations of Na+ or to pH 8.0–9.0 provided evidence for the involvement of a plasma membrane Na+/H+ antiporter and a correlation between intracellular Na+ levels and pressure resistance. The results support the hypothesis that moderate high pressure causes indirect cell death in R. rubra by inducing cytosolic acidification. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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