Chloride channels in basolateral TAL membranes. XVIII. Phenylglyoxal induces functional mcCIC-Ka activity in basolateral MTAL membranes.
| Autor: | Winters, C. J., Andreoli, T. E. |
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| Předmět: |
CELL culture
ION channels MESSENGER RNA ENZYME kinetics BIOLOGICAL membranes LYSINE LIPID metabolism POTASSIUM metabolism ADENOSINE triphosphate ALDEHYDES ANIMAL experimentation BIOLOGICAL transport CELL membranes CELLULAR signal transduction CHLORIDES COMPARATIVE studies ENZYME inhibitors KIDNEYS RESEARCH methodology MEDICAL cooperation MEMBRANE proteins BASAL lamina MICE POTASSIUM RABBITS RESEARCH RESEARCH funding TRANSFERASES EVALUATION research PHARMACODYNAMICS |
| Zdroj: | Journal of Membrane Biology; Sep2003, Vol. 195 Issue 2, p63-71, 9p |
| Abstrakt: | Cultured mouse MTAL cells contain more mRNA encoding the Cl- channel mcCIC-Ka, which mediates CTAL Cl- absorption, than mRNA encoding the Cl- channel mmCIC-Ka, which mediates MTAL Cl- absorption. mmCIC-Ka and mcCIC-Ka have three functional differences: 1) mmCIC-Ka open time probability, Po, increases with increasing cytosolic Cl-, but variations in cytosolic Cl- do not affect Po in mcCIC-Ka; 2) mmCIC-Ka is gated by (ATP + PKA), while (ATP + PKA) have no effect on Po in mcCIC-Ka; and 3) mmCIC-Ka channels have single-ion occupancy, while mcCIC-Ka channels have multi-ion occupancy. Using basolateral vesicles from MTAL cells fused into bilayers, we evaluated the effects of 1 mM cytosolic phenylglyoxal (PGO), which binds covalently to lysine or arginine, on Cl- channels. With PGO pretreatment, Cl- channels were uniformly not gated either with increases in cytosolic-face Cl- or with (ATP + PKA) at 2 mM cytosolic-face Cl-; and they exhibited multi-ion occupancy kinetics typical for mcCIC-Ka channels. Thus, in basolateral MTAL membranes, blockade of Cl- access to arginine or lysine residues on mmCIC-Ka by PGO results in Cl- channels having the functional characteristics of mcCIC-Ka channels. [ABSTRACT FROM AUTHOR] |
| Databáze: | Complementary Index |
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