| Autor: |
Soh, Beatrice W., Ooi, Zi-En, Vissol-Gaudin, Eleonore, Leong, Chang Jie, Hippalgaonkar, Kedar |
| Předmět: |
|
| Zdroj: |
Lab on a Chip; 8/21/2023, Vol. 23 Issue 16, p3716-3726, 11p |
| Abstrakt: |
In this work, we present an automated platform for trapping and stretching individual micro- and nanoscale objects in solution using electrokinetic forces. The platform can trap objects at the stagnation point of a planar elongational electrokinetic field for long time scales, as demonstrated by the trapping of <100 nm polystyrene beads and DNA molecules for minutes, with a standard deviation in displacement from the trap center <1 μm. This capability enables the stretching of deformable nanoscale objects in a high-throughput fashion, as illustrated by the stretching of more than 400 DNA molecules within ∼4 hours. The flexibility of the electrokinetic stretcher opens up numerous possibilities for complex manipulation, with sequential stretching of a molecule at different voltages and multiple stretch–relaxation cycles of the same molecule as examples. The platform described provides an automated, high-throughput method to track and manipulate objects for real-time studies of micro- and nanoscale systems. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
