| Abstrakt: |
Long-term cultivation of two lines of human MSCs isolated from different places in the placenta was carried out. The MSC-PL-1 cell line is characterized by premature aging compared to the MSC-PL-2 line. Upon induction of adipogenic differentiation in cells of both lines, it turned out that it does not occur at early and late passages in MSC-PL-1 cells, in contrast to MSC-PL-2 cells. Comparative analysis of the activities of matrix metalloproteinases (MMPs) 1, 2, and 9 during replicative aging (RA) of these lines indicates interstrain differences. Thus, in the MSC-PL-2 line, there is a decrease in the levels of MMP-2 and -1 activity during RA and the level of MMP-9 activity does not change, while, in the MSC-PL-1 line, which has premature RA, an increase in the level of MMP-1 and -9 activity and a decrease in the level of MMP-2 activity are observed. Analysis of the activities of MMP-1 and -2 during adipogenic differentiation in MSC-PL-2 cells at the early sixth passage showed a number of differences between them within 21 days, but the changes in both MMPs vary. MMP-9 activity during 21 days of differentiation changes differently. At the late 16th passage, the nature of changes in the activity of all three MMPs during 21 days of differentiation is the same. Due to the absence of adipogenic differentiation in MSC-PL-1 cells, we analyzed the activities of MMP-1, -2, and -9 when cultivated in an induction medium for 21 days at early (sixth) and late (13th) passages. In both variants, there are changes in the activity of three MMPs during cultivation in the induction medium, but these changes are not synchronous. The activities of all three MMPs at the 13th passage decrease relative to the sixth passage. In general, the results obtained indicate the participation of MMPs in a wide range of processes in MSCs. [ABSTRACT FROM AUTHOR] |
Full text from SpringerLink