| Autor: |
Sombié, Ernest N., Somda, Donzèo G., Konaté, Soumaila, Youl, Ollo, Traoré, Tata K., N'do, Jotham Y-P., Belem-Kabré, Wendkouni L. M. E., Ouédraogo, Geoffroy G., Ouédraogo, Noufou, Hilou, Adama |
| Předmět: |
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| Zdroj: |
Tropical Journal of Natural Product Research; Jul2023, Vol. 7 Issue 7, p3462-3469, 8p |
| Abstrakt: |
Calotropis procera (Ait.) R.Br (Apocynaceae) is a medicinal plant known for its multiple therapeutic virtues including the management of liver diseases in Burkina Faso. This study aimed to evaluate the hepato-protective properties of the fractions of the ethanol extract of Calotropis procera roots barks while identifying chemical compounds in these extracts. Sterols, triterpene and phenolic compounds were sought and quantified in the fractions of the ethanol extract of Calotropis procera root bark by HPLC-DAD. The anti-hepatotoxic activity of fractions was evaluated using diethylnitrosamine (DEN) to induce acute liver inflammation in male Wistar rats. Serum biomarkers, alanine transaminase (ALAT), aspartate transaminase (ASAT), alkaline phosphatase (ALP), total proteins and Albumin were evaluated and the activities of antioxidant enzymes (Superoxide dismutase and catalase) as well as the level of malondialdehyde (MDA) were determined in the liver homogenate. The histopathological study of sections of livers pretreated with the fractions was also carried out. The evaluation of the anti-hepatotoxic activity showed that the n-butanol fraction and residual aqueous fraction had best effects on serum biomarkers (ALAT, ASAT, ALP) as well as on the activities of the antioxidant enzymes Superoxide dismutase and catalase and on hepatic tissue. Gallic acid, an antioxidant, antihepatotoxic, antifibrotic compound was identified in significant quantities in these two fractions at the respective contents of 17.92 ± 0.26 µg/mg and 18.76 ± 0.27 µg/mg of dry extract, suggesting that this compound would participates in the hepatoprotective properties of these extracts. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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