Biochemical Characterization of the Yeast Yarrowia lipolytica Overproducing Carboxylic Acids from Ethanol: Nitrogen Metabolism Enzymes.

Autor:	Il'chenko, A. P., Chernyavskaya, O. G., Shishkanova, N. V., Finogenova, T. V.
Předmět:	ENZYMES CITRIC acid NAD (Coenzyme) GLUTAMATE dehydrogenase NITROGEN GLUTAMINE synthetase
Zdroj:	Microbiology (00262617); Jul2003, Vol. 72 Issue 4, p418-422, 5p
Abstrakt:	A comparative assay of nitrogen metabolism enzymes in the Yarrowia lipolytica mutant N1 grown under conditions promoting the overproduction of either α-ketoglutaric acid (KGA) or citric acid showed that the overproduction of KGA correlates with an increase in the activities of the NAD- and NADP-linked glutamate dehydrogenase, glutamic–pyruvic transaminase, and glutamic–oxaloacetic transaminase reactions. These reactions are likely to be responsible for the overproduction of KGA by this mutant. In contrast, the overproduction of citric acid correlated with a decline in the activities of the NAD- and NADP-linked glutamate dehydrogenases and with an increase in the activities of glutamine synthetase and glutamate synthase. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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