| Autor: |
Matoba, Shogo, Kanai, Yoshiakira, Kidokoro, Tomohide, Kanai-Azuma, Masami, Kawakami, Hayato, Hayashi, Yoshihiro, Kurohmaru, Masamichi |
| Předmět: |
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| Zdroj: |
Journal of Cell Science; 4/1/2005, Vol. 118 Issue 7, p1449-1459, 11p, 13 Diagrams, 1 Chart, 1 Graph |
| Abstrakt: |
Sry is transiently activated in pre-Sertoli cells of the gonadal ridge to initiate testis differentiation in mice. In pre-Sertoli cells, however, the cellular events induced immediately after the onset of Sq expression remain largely unknown. Here we show that testis-specific glycogen accumulation in pre-Sertoli cells is one of the earliest cellular events downstream of Sq action. In developing XY gonads, glycogen accumulation starts to occur in pre-Sertoli cells from around 11.15 dpc (tail somite 14 stage) in a center-to-pole pattern similar to the initial Sq expression profile. Glycogen accumulation was also found in XX male gonads of Sry-transgenic embryos, but not in XX female gonads of wildtype embryos at any developmental stage. In vitro analyses using various culture conditions suggest that testis-specific glycogen deposition is a tissue-autonomous event that can be induced even in serum-free conditions and in a culture of gonadal explants without adjacent mesonephros. Moreover, glycogen accumulation in pre-Sertoli cells was significantly inhibited in vitro by the PI3K inhibitor LY294002, but not by the MEK inhibitor PD98059. Active phospho-AKT (PI3K effector) showed a high degree of accumulation in gonadal somatic cells of genital ridges in a testis-specific manner, both in vitro and in vivo. Therefore, these findings suggest that immediately after the onset of Sry expression, activation of the PI3K-AKT pathway promotes testis- specific glycogen storage in pre-Sertoli cells. To the best of our knowledge, this is a novel Sty-downstream cellular event which preserves this readily available energy source in Sertoli cells for testis-specific morphogenesis and hormone production. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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