Molecular and Serological Characterization of Group A Rotavirus Isolates Obtained from Hospitalized Children in Goiânia, Brazil, 1998–2000.

Autor: Souza, M. B. L. D., Rácz, M. L., Leite, J. P. G., Soares, C. M. A., Martins, R. M. B., Munford, V., Cardoso, D. D. P.
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Zdroj: European Journal of Clinical Microbiology & Infectious Diseases; Jul2003, Vol. 22 Issue 7, p441-443, 3p
Abstrakt: Fecal samples positive for rotavirus group A (n=120) were analyzed by enzyme immunoassay-monoclonal antibody (EIA-MAb) serotyping and/or reverse transcription/multiplex polymerase chain reaction (PCR) amplification to determine the prevalence of the [P] and G genotypes. The most prevalent G genotype/serotype detected was G1 (76.7%), followed by G2 (5.0%). Six samples were characterized as G9 by multiplex PCR, and one sample was characterized as G3 by EIA-MAb. The combinations of [P] and G genotypes found were P[8] and G1 (20.8%), P[6] and G1 (10.8%), P[6] and G9 (4.2%), P[8] and G2 (1.7%), and P[6] and G2 (0.8%). The diversity of rotavirus group A [P] and G genotypes/serotypes reinforces the need for continuous characterization of rotaviruses circulating in populations in Brazil. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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