| Autor: |
González-Brusi, L., Pérez-Gómez, A., Quiroga, A. C., Tamargo, C., Ramos-Ibeas, P., Bermejo-Álvarez, P. |
| Předmět: |
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| Zdroj: |
Reproduction, Fertility & Development; 2023, Vol. 35 Issue 12, p614-621, 8p |
| Abstrakt: |
Context: Arachidonic acid (AA) is the precursor of prostaglandins, which may play autocrine roles during early embryo development. Aims: To test the developmental effects of addition of AA to pre- and post-hatching culture media on in vitro –produced bovine embryos. Methods: Pre-hatching effects of AA were tested by culturing bovine zygotes in synthetic oviductal fluid (SOF) supplemented with 100 or 333 μM AA. Post-hatching effects of AA were tested by culturing Day 7 blastocysts in N2B27 supplemented with 5, 10, 20 or 100 μM AA up to Day 12. Key results: Pre-hatching development to blastocyst was completely abrogated at 333 μM AA, whereas blastocyst rates and cell numbers were not altered at 100 μM AA. Impaired post-hatching development was observed at 100 μM AA, whereas no effect on survival rates was noted at 5, 10 and 20 μM AA. However, a significant reduction in Day 12 embryo size was observed at 10 and 20 μM AA. Hypoblast migration, epiblast survival and formation of embryonic-disc-like structures were unaffected at 5–10 μM AA. AA exposure downregulated the genes PTGIS , PPARG , LDHA and SCD in Day 12 embryos. Conclusions: Pre-hatching embryos are mostly irresponsive to AA, whereas AA was observed to have negative effects during early post-hatching development. Implications: AA does not improve in vitro bovine embryo development and is not required up to early post-hatching stages. Arachidonic acid (AA) is the precursor of prostaglandins, which have been suggested to play autocrine roles during early development. In this study, we tested the developmental effect of the addition of AA to pre- and post-hatching bovine embryo culture, observing that pre-hatching embryos are mostly irresponsive to AA, whereas early post-hatching in vitro development is negatively affected by AA. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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