Microscale Recovery of Total Lipids from Fish Tissue by Accelerated Solvent Extraction.

Autor: Dodds, Eric D., McCoy, Mark R., Geldenhuys, Adeline, Rea, Lorrie D., Kennish, John M.
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Zdroj: Journal of the American Oil Chemists' Society (JAOCS); Sep2004, Vol. 81 Issue 9, p835-840, 6p, 4 Charts, 3 Graphs
Abstrakt: A number of techniques are available for the extraction of lipids from a variety of tissues; however, conventional methods are characteristically labor intensive, typically involve large volumes of toxic solvents, and usually require at least 1 g of tissue. With the availability of accelerated solvent extraction (ASE) technology, the opportunity exists to modify classical lipid extraction techniques such that automated high-pressure, high-temperature extractions may be performed with the use of far smaller volumes of costly and harmful solvents. Moreover, the high extraction efficiency attainable by ASE suggests that significantly less tissue would be required than is routinely used. This paper describes the adaptation of previously developed lipid extraction solvent systems for use with ASE toward the purpose of extracting total lipids from 100 mg of fish tissue. The efficacy of three solvent systems for lipid extraction from representative fish tissues, including a standard reference material, was explored using gravimetry and FA analysis by GC. A TG was used as a surrogate to monitor overall method performance. The findings herein demonstrate that microscale ASE represents an effective and efficient alternative to traditional lipid extraction techniques based on quantity and composition of extracted lipid, surrogate recovery, and precision. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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