| Autor: |
Xue-jun, Cao, Xing-yan, Wu, Fonseca, Luis J.P., Cabral, Joaquim M.S., xE3;o Carlos#Marcos, Jo& |
| Předmět: |
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| Zdroj: |
Biotechnology Letters; Jan2004, Vol. 26 Issue 2, p97-101, 5p |
| Abstrakt: |
Bioconversion of penicillin G in PEG 20000/dextran T 70 aqueous two-phase systems was achieved using the recombinant Escherichia coli A56 (ppA22) with an intracellular penicillin acylase as catalyst. The best conversion conditions were attained for: 7% (w/v) substrate (penicillin G), enzyme activity in bottom phase 52 U ml-1, pH 7.8, temperature 37 °C, reaction time 40 min. Five repeated batches could be performed in these conditions. Conversions ratios between 0.9–0.99 mol of 6-aminopenicillanic acid (6-APA) per mol of penicillin G, were obtained and volumetric productivity was 3.6–4.6 μmol min-1 ml-1. In addition the product 6-APA could be directly crystallized from the top phase with a purity of 96%. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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