Characterization of a gene highly expressed in the Brassica napus pistil that encodes a novel proline-rich protein.

Autor: Foster, Elizabeth, Lévesque-Lemay, Madeleine, Schneiderman, Danielle, Albani, Diego, Schernthaner, Johann, Routly, Elizabeth, Robert, Laurian
Předmět:
Zdroj: Sexual Plant Reproduction; Mar2005, Vol. 17 Issue 6, p261-267, 7p
Abstrakt: Pis 30, a gene highly expressed in Brassica napus pistils and encoding a novel proline-rich protein was isolated and characterized. Sequences homologous to the Brassica Pis 30 gene were found only in Arabidopsis thaliana. The Pis 30 gene encodes a mature protein of 8.4 kDa with no previously characterized protein domains and whose function remains unknown. PIS 30 contains especially high levels of Pro (33%), but also of Leu (14%), Phe (10%) and Ser (6%). Although it is a proline-rich protein, PIS 30 shows only limited similarity to previously characterized plant proline-rich proteins. When compared to the stigma-specific activity of the B. napus SLR1 gene promoter in pistils of transgenic Arabidopsis, an 808 bp Pis 30 promoter fragment directed ß-glucuronidase expression primarily in the ovary, as well as in the stigma. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index