| Autor: |
Wels, W., Baldrich, M., Chakraborty, T., Gross, R., Goebel, W. |
| Předmět: |
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| Zdroj: |
Molecular Microbiology; Sep1992, Vol. 6 Issue 18, p2651-2659, 9p, 3 Black and White Photographs, 3 Diagrams, 1 Chart, 1 Graph |
| Abstrakt: |
We have studied the expression of the germ fragments encoding the enzymatically active portion of three bacterial cytotoxins: exotoxin A (ETA) of Pseudomonas aeruginosa, and pertussis toxin (PT) and adenylate cyclase toxin (CYA) of Bordetella pertussis, in sensitive mammalian target cells. Expression of active ETA and CYA was lethal to the producing cells and stable transfectants of Cos-1 cells containing the corresponding genes could not be obtained. The expression of the PTS1 subunit was tolerated by the producing mammalian ceils. Since PT is cytotoxic because of ADP-riboaylation of G-proteins, we assume that the endogenously expressed PTS1 may not find the cellular target G proteins or PTS1 alone may not be sufficient for ADP-ribosylation of these proteins in vivo. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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