| Autor: |
Andres-Alonso, Maria, Borgmeyer, Maximilian, Mirzapourdelavar, Hadi, Lormann, Jakob, Klein, Kim, Schweizer, Michaela, Hoffmeister-Ullerich, Sabine, Oelschlegel, Anja M., Dityatev, Alexander, Kreutz, Michael R. |
| Zdroj: |
Cell Reports; Jul2023, Vol. 42 Issue 7, pN.PAG-N.PAG, 1p |
| Abstrakt: |
The complex cytoarchitecture of neurons poses significant challenges for the maturation of synaptic membrane proteins. It is currently unclear whether locally secreted synaptic proteins bypass the Golgi or whether they traffic through Golgi satellites (GSs). Here, we create a transgenic GS reporter mouse line and show that GSs are widely distributed along dendrites and are capable of mature glycosylation, in particular sialylation. We find that polysialylation of locally secreted NCAM takes place at GSs. Accordingly, in mice lacking a component of trans -Golgi network-to-plasma membrane trafficking, we find fewer GSs and significantly reduced PSA-NCAM levels in distal dendrites of CA1 neurons that receive input from the temporoammonic pathway. Induction of long-term potentiation at those, but not more proximal, synapses is severely impaired. We conclude that GSs serve the need for local mature glycosylation of synaptic membrane proteins in distal dendrites and thereby contribute to rapid changes in synaptic strength. [Display omitted] • Golgi satellites (GSs) have features of TGN membranes and are widely distributed • De novo glycosylation, including sialylation, occurs at Golgi satellites • GSs are essential for NCAM polysialylation at distal dendrites of pyramidal neurons • LTP expression is impaired in distal dendrites with fewer GSs and reduced PSA-NCAM Maturation of locally secreted proteins is challenged by the polarized morphology of neurons and the somatic localization of the Golgi apparatus. Andres-Alonso et al. show that dendritic Golgi satellites are capable of complex glycosylation and that polysialylation of NCAM at these organelles is essential for long-term plasticity at distal synapses. [ABSTRACT FROM AUTHOR] |
| Databáze: |
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