Phase intensity nanoscope (PINE) opens long-time investigation windows of living matter.

Autor: Cui, Guangjie, Liu, Yunbo, Zu, Di, Zhao, Xintao, Zhang, Zhijia, Kim, Do Young, Senaratne, Pramith, Fox, Aaron, Sept, David, Park, Younggeun, Lee, Somin Eunice
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Zdroj: Nature Communications; 7/18/2023, Vol. 14 Issue 1, p1-10, 10p
Abstrakt: Fundamental to all living organisms and living soft matter are emergent processes in which the reorganization of individual constituents at the nanoscale drives group-level movements and shape changes at the macroscale over time. However, light-induced degradation of fluorophores, photobleaching, is a significant problem in extended bioimaging in life science. Here, we report opening a long-time investigation window by nonbleaching phase intensity nanoscope: PINE. We accomplish phase-intensity separation such that nanoprobe distributions are distinguished by an integrated phase-intensity multilayer thin film (polyvinyl alcohol/liquid crystal). We overcame a physical limit to resolve sub-10 nm cellular architectures, and achieve the first dynamic imaging of nanoscopic reorganization over 250 h using PINE. We discover nanoscopic rearrangements synchronized with the emergence of group-level movements and shape changes at the macroscale according to a set of interaction rules with importance in cellular and soft matter reorganization, self-organization, and pattern formation. The authors present phase intensity nanoscope (PINE), which uses an integrated phase-intensity multilayer thin film to localize randomly distributed nanoprobes and resolve sub-10 nm cellular architectures. They demonstrate dynamic imaging of nanoscopic reorganization over 250 h and find that nanoscale rearrangements emerging into macroscale rearrangements are synchronized. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index