| Autor: |
Barik, Pabitra, Saharan, Neelam, Krishnani, Kishore Kumar, Vardia, Hemendra Kumar |
| Předmět: |
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| Zdroj: |
Journal of Experimental Zoology India; Jul2023, Vol. 26 Issue 2, p1437-1443, 7p |
| Abstrakt: |
Aquaculture is one of the most rapidly growing sectors. The utilization of antimicrobials for aquaculture is expanding with intensification and contributing progressively to the spread of antimicrobial resistance (AMR). Also, water scarcity demands the use of wastewater in aquaculture. This study aims to synthesize copper nanoparticles (NPs) using Bacillus megaterium isolated from mangrove soils, which is expected to simplify the use of wastewater in aquaculture. Copper NPs were synthesized using B. megaterium and characterized by DLS-zeta potential, UV-vis, XRD, FTIR, and TEM analysis. The synthesized NPs were evaluated for antimicrobial activity and ex-situ tests were performed to determine their efficacy in removing ammonia from wastewater intended for aquaculture purposes. B. megaterium was isolated and was identified by 16S rRNA gene PCR-amplified sequences. DLS-zeta potential, UV-vis, XRD, FTIR and TEM analysis revealed the quality of synthesized NPs. The mean zeta potential of the biosynthesized NPs was <-5.65 mV and had an average size of 6.8 nm. The antimicrobial test revealed that copper NPs inhibited the growth of microorganisms and the inhibitory zone was ~30 mm. Also, the ammonia concentration in wastewater treated with copper NPs reduced to less than 0.05 mg/L from 10 mg/L. These findings suggest that synthesized copper NPs from B. megaterium significantly inhibited microbial activity. Moreover, NPs efficiently removed ammonia from wastewater intended for fish culture. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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