| Autor: |
Maus, Kenneth D., Stephenson, Daniel J., Macknight, H. Patrick, Vu, Ngoc T., Hoeferlin, L. Alexis, Kim, Minjung, Diegelmann, Robert F., Xie, Xiujie, Chalfant, Charles E. |
| Předmět: |
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| Zdroj: |
Science Signaling; 7/11/2023, Vol. 16 Issue 793, p1-19, 19p |
| Abstrakt: |
Uncontrolled inflammation is linked to poor outcomes in sepsis and wound healing, both of which proceed through distinct inflammatory and resolution phases. Eicosanoids are a class of bioactive lipids that recruit neutrophils and other innate immune cells. The interaction of ceramide 1-phosphate (C1P) with the eicosanoid biosynthetic enzyme cytosolic phospholipase A2 (cPLA2) reduces the production of a subtype of eicosanoids called oxoeicosanoids. We investigated the effect of shifting the balance in eicosanoid biosynthesis on neutrophil polarization and function. Knockin mice expressing a cPLA2 mutant lacking the C1P binding site (cPLA2αKI/KI mice) showed enhanced and sustained neutrophil infiltration into wounds and the peritoneum during the inflammatory phase of wound healing and sepsis, respectively. The mice exhibited improved wound healing and reduced susceptibility to sepsis, which was associated with an increase in anti-inflammatory N2-type neutrophils demonstrating proresolution behaviors and a decrease in proinflammatory N1-type neutrophils. The N2 polarization of cPLA2αKI/KI neutrophils resulted from increased oxoeicosanoid biosynthesis and autocrine signaling through the oxoeicosanoid receptor OXER1 and partially depended on OXER1-dependent inhibition of the pentose phosphate pathway (PPP). Thus, C1P binding to cPLA2α suppresses neutrophil N2 polarization, thereby impairing wound healing and the response to sepsis. Editor's summary: Neutrophils are recruited to sites of injury and infection by various factors, including lipids called eicosanoids, and can adopt a proinflammatory N1 phenotype or an anti-inflammatory N2 phenotype. Maus et al. found that an eicosanoid subtype called oxoeicosanoids enhanced wound healing and reduced the susceptibility to sepsis in mice due to an increase in N2 neutrophil recruitment. Oxoeicosanoids promoted N2 polarization through autocrine activation of the oxoeicosanoid receptor OXER1. These findings suggest potential strategies for manipulating neutrophil polarization to improve clinical outcomes. —Annalisa M. VanHook [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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