| Abstrakt: |
The study was to establish a fast and accurate ultra performance liquid chromatography-tandem mass spectrometry method for determination of DON in feed. The samples were extracted with 10 mL water, then oscillation and exocentric, purified by fungus toxin purification column, filtered by 0.22 μm membrane, determined by UPLC-MS/ MS. When DON supplementation levels were 0.01, 0.05 and 0.10 μg/kg, the recoveries were 83.92%~94.65% (concentrate feed), and RSD were 6.35%~7.09%. The DON had good linear relationships in their linear ranges, the linear coefficient was greater than 0.999 0, the limit of detection was 0.01 mg/kg. DON was detected in 194 of 216 batches of feed samples, and the detection rate was 89.8%. The study indicates that the method is easy to operate, low cost, high accuracy and can meet the requirements of large-scale detection of feed samples. [ABSTRACT FROM AUTHOR] |
