Low density lipoproteins modulate collagen metabolism in fibroblasts.
| Autor: | Joseph, Jacob, Ranganathan, Subramanian, Mehta, Jawahar L. |
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| Předmět: |
LOW density lipoproteins
METABOLIC disorders COLLAGEN FIBROBLASTS METALLOPROTEINASES PROTEINS RNA metabolism CELL lines CELL receptors COMPARATIVE studies DOSE-effect relationship in pharmacology HEPARIN IMMUNOBLOTTING RESEARCH methodology MEDICAL cooperation NUCLEOTIDE separation PROTEOLYTIC enzymes RESEARCH SKIN WESTERN immunoblotting EVALUATION research PHARMACODYNAMICS METABOLISM |
| Zdroj: | Journal of Cardiovascular Pharmacology & Therapeutics; Jun2003, Vol. 8 Issue 2, p161-166, 6p |
| Abstrakt: | High levels of low-density lipoprotein are associated with atherosclerosis, and myocardial and arterial remodeling. We postulated that low-density lipoprotein influences collagen synthesis and degradation in fibroblasts as a potential mechanism of tissue remodeling. Incubation of cultured human skin fibroblasts with low-density lipoproteins resulted in a time-dependent and dose-dependent increase in the secretion of matrix metalloproteinase activity measured by gelatin zymography. Western blot analysis showed a concomitant increase in matrix metalloproteinase-1 protein. Northern blot analysis demonstrated an increase in collagen I messenger RNA after treatment with low-density lipoprotein. The matrix metalloproteinase-1 secretory response of fibroblasts to low-density lipoprotein was attenuated by heparin, which inhibits low-density lipoprotein uptake through the low-density lipoprotein-receptor. These observations suggest that low-density lipoprotein has a regulatory effect on collagen metabolism in fibroblasts. [ABSTRACT FROM AUTHOR] |
| Databáze: | Complementary Index |
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