| Autor: |
Tvede, N., Pedersen, B. K., Hansen, F. R., Bendix, T., Christensen, L. D., Galbo, H., Halkjæ-Kristensen, J. |
| Předmět: |
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| Zdroj: |
Scandinavian Journal of Immunology; Mar1989, Vol. 29 Issue 3, p383-389, 7p |
| Abstrakt: |
The present study was designed to examine the effect of physical exercise on subsets and proliferative responses of blood mononuclear cells. Sixteen young, healthy volunteers underwent 60 min of bicycle exercise at 75% of maximal oxygen uptake (VO2max). After an interval of at least 1 week, six of the subjects underwent a 60-min back muscle training period at up to 30% of and 24 h later. Blood mononuclear cell (BMNC) subpopulations were determined and the proliferative responses after incubation with phytohaemagglutimin (PHA) or purified derivative of tuberculin (PPD), were quantified by [³H]thymidine incorporation. During bicycle exercise the relative blood concentration of T cells (CD3+ cells) declined, mainly due to a fall in T helper cells (CD4+ cells). the natural killer (NK) cell subset (CD16+ cells) increased during work, but reverted after, the monocytes (CD14+ cells) increased 2 h after work, whereas the B-cell subset (CD20+ cells) did not change. BMNC subsets were not significantly changed by back muscle exercise. The PHA-induced proliferative response decreased during bicycle exercise, whereas the PPD-induced response did not change. No significant changes occurred during back muscle exercise. Investigation of subgroups after incubation with [³H]thymidine showed that the proliferative response per CD4+ cell did not change in relation to exercise, but the contribution of the CD4+ subgroup to proliferation declined during bicycle exercise due to the decreased proportion of CD4+ cells. The suppression of the PHA response during bicycle exercise can be explained in part by a relative fall in CD4+ cells. The pool sizes BMNC subfraction may be elicited by increased catechalamibe and cortisol levels. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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