| Autor: |
Hensley, Nicole, Elliott, Elizabeth R., Abul-Khoudoud, Maya O., Cooper, Robin L. |
| Předmět: |
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| Zdroj: |
Applied Biosciences; Jun2023, Vol. 2 Issue 2, p236-248, 13p |
| Abstrakt: |
Highlights: 2-APB slowed heart rate at a high concentration (>10 µM) in the slo mutant line. The slo strain and CS strain both increased heart rate when exposed to 5-HT. The slo strain and CS strain both increased heart rate when exposed to 5-HT following 2-APB incubation. No differences were measured between the use of thapsigargin or 2-APB on heart rate with exposure to 5-HT between the slo and CS strain. Cardiac contractile cells depend on calcium in order to function. Understanding the regulation of calcium influx, efflux, and release from the sarcoplasmic reticulum is essential. The focus of this investigation is to address how a reduction of functional Ca2+-activated K+ (KCa) channels, via a mutational line, might impact the heart rate in larva when the SER is also modulated through Ca2+ loading and stimulation. The larval heart tube is exposed in situ and flushed with saline. With a known saline composition, a potential therapeutic pharmacological agent, 2-Aminoethyl diphenylborinate (2-APB), was examined for its effect on heart rate, as well as to determine the contribution from KCa channels. In this study, it was determined that mutation in the K(Ca) channel (i.e., Slo) showed a different trend than the wild-type CS strain. Exposure to high concentrations of 50 µM 2-APB decreased heart rate in the Slo strain and increased it in the wild-type CS strain. Serotonin increased heart rate in both thapsigargin- and 2-APB-treated larvae, with no significant difference between the strains. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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