| Autor: |
Lang, S. M., Heinzlmann, M., Stratakis, D. F., Teschauer, W., Loeschke, K. |
| Předmět: |
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| Zdroj: |
American Journal of Gastroenterology (Springer Nature); Dec1997, Vol. 92 Issue 12, p2166-2170, 5p, 1 Black and White Photograph, 2 Diagrams, 2 Charts |
| Abstrakt: |
Objectives: The goal of this study was the early detection of malignant transformation in patients with longstanding ulcerative colitis; therefore, mutations of the Ki-ras and p53 gene were analyzed in lavage solution and biopsies obtained at surveillance colonoscopy. Methods: DNA was isolated from 14 patients (nine female, five male) with a history of pancolitis for more than 10 yr. Exon 1 of the Ki-ras gene and exons 5-8 of the p53 gene were amplified via polymerase chain reaction. Mutations of the p53 gene were detected via single-strand conformation polymorphism analysis; point mutations of the Ki-ras gene were hybridized on dot blots with oligonucleotides marked with digoxigenin, Results: Wild-type Ki-ras and wild-type p53 were detected in all cases of ulcerative colitis and in four of ,seven control patients. In two ulcerative colitis patients, a mutation was found in the Ki-ras gene (Gly →, Asp 12 and Gly → Val 12), and in one patient, a mutation in exon 5 of the p53 gene. Mutations were found only in the lavage fluid, whereas random biopsies were negative. Conclusions: From colonic lavage fluid, it is possible to extract DNA of sufficient quantity and quality for polymerase chain reaction-based amplification and subsequent analysis via single-strand conformation polymorphism or hybridization. Mutations were found in three of 14 patients with long-standing ulcerative colitis but were not found in controls. The method may be useful for the screening of such patients. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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