| Autor: |
Kamaladini, Hossein, Mohkami, Zaynab, Salami, Robab, Khajeh, Hamideh, Fazeli-Nasab, Bahman |
| Předmět: |
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| Zdroj: |
Gene, Cell & Tissue; Apr2023, Vol. 10 Issue 2, p1-9, 9p |
| Abstrakt: |
Background: Contamination of tissue culturemediumis acommonproblem that causes the loss of someor all cultures. Despite all measures taken in tissue culture laboratories to prevent culture contamination, bacterial and fungi contamination is still a significant problem under in vitro culture. Achieving a suitable method for controlling the contaminants in the in vitro culture medium will be a considerable development in the micropropagation of dates. Objectives: We aimed to investigate the effects of different aqueous and ethanolic extracts of Rhazya stricta on the disinfection of dates under in vitro culture. Methods: A factorial test was performed based on a completely randomized design with three replications. The study included three factors: plant organs (leaves and roots), solvent type (ethanolic and aqueous), and extract concentration (0, 25, 50, and 75 mg/mL). Results: The lowest contamination (38.33%) was seen in the treatment with ethanolic root extract (50 ppm). In comparison, the control and the treatment containing aqueous leaf extract (with concentrations of 0and 25ppm)resulted in the highest contamination rate (100%) in tissue culture conditions. Conclusions: The alcoholic extract of medicinal plants can be used to disinfect the explants and the substrate. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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