| Autor: |
Nawi, Nuraini, Majid, Fadzilah Adibah Abdul, Amin, Nakisah Mat |
| Předmět: |
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| Zdroj: |
AIP Conference Proceedings; 2023, Vol. 2738 Issue 1, p1-6, 6p |
| Abstrakt: |
Breast cancer is the most cancer which had spread among woman worldwide. The current treatment showed the resistance of cancer toward anti-cancer drug. Acanthamoeba are free-living protozoa and can be found everywhere such as fresh water, in brackish water, sea water and even in contact lens. Acanthamoeba is known as pathogenic organism toward human and caused several diseases in human such as eye keratitis and fatal granulomatous encephalitis. Previous studies showed the pathogenic free-living Acanthamoeba lysate interfering in intercellular signalling of the host by inducing morphological features of apoptosis in tumor cells. Thus this study was conducted to explore the potential of Acanthamoeba as anti-cancer drug source. In this study, anti-cancer activities of Acanthamoeba were determined by extraction of 3 strains of Acanthamoeba lysate and conducting cytotoxicity assays. MTT assay was used to determine the cytotoxicity of Acanthamoeba lysate toward MCF 7 cells. The modes of cells death were conducted through DNA fragmentation and AOPI assays. The exposure of MCF 7 cells with Acanthamoeba lysate showed an effect on the viability of MCF 7 cells. Acanthamoeba sp (SW isolate) was found to be most cytotoxic against MCF 7 cells compared to others, with IC50 for Acanthamoeba sp. (SW isolate) was 15.09 ± 1.12b µg/ml. The other two strains of Acanthamoeba did not showed significant different, with IC50 for Acanthamoeba castelleanii (IMR isolate) was 31.46 ± 1.10a µg/ml and Acanthamoeba sp (HKL isolate) was 28.41 ± 1.13a µg/ml. MCF 7 cells treated with Acanthamoeba lysate showed a normal nucleosomal fragment pattern due to endonucleolytic cleavage of DNA into multiple nucleosome size units. MCF 7 cells showed morphological characteristic of apoptosis such as cell membrane blebbing which could be clearly seen through AOPI assays. In conclusion, through this study our funding indicates Acanthamoeba lysate to have potential cytotoxic effect on breast cancer. More study need to be conducted to select and identify the bioactive compound with anti-cancer activity in the lysate of Acanthamoeba spp. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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