| Autor: |
Bhide, Gaurang P, Bohrer, Natalie E, Song, Danying, Cabel, Jeanne, Lake, Marc R, Harlan, John E, Miesbauer, Laura J |
| Předmět: |
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| Zdroj: |
Glycobiology; May2023, Vol. 33 Issue 5, p364-368, 5p |
| Abstrakt: |
Monoclonal antibodies comprise a major class of biologic therapeutics and are also extensively studied in immunology. Given the importance of glycans on antibodies, fluorescent labeling of enzymatically released glycans and their LC/MS analysis is routinely used for in-depth characterization of antibody glycosylation. In this technical note, we propose a method for facile characterization of glycans in the variable region of antibodies using sequential enzymatic digests with Endoglycosidase-S2 and RapidTM Peptide-N-Glycosidase-F followed by labeling with a fluorescent dye carrying an NHS-carbamate moiety. The results and proposed mechanism also suggest that the choice of glycosidases along with the labeling chemistry is critical for accurate glycan analysis for a desired application. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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