| Autor: |
Hasan, Zainab Yaseen Mohammed, Alsamarrae, Khulood W., Rushdi, Ahmed |
| Předmět: |
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| Zdroj: |
Journal of Contemporary Medical Sciences; Mar/Apr2023, Vol. 9 Issue 2, p101-105, 5p |
| Abstrakt: |
Objectives: The study aimed to investigate the effects of Iraqi Lycium barbarm carotene on normal human blood lymphocytes at molecular level. Methods: The normal human blood lymphocytes culture was exposed to Carotene extracted from Iraqi Lycium barbarm to estimate the interleukins expression in treated lymphocytes represented by IL-10 and TNF-α as a moderator of immune response at a molecular scale, and their CD markers changes expression (CD3, CD4 & CD8) by flow-cytometer apparatus. Results: Total carotenes content was 0.33 mg/g powdered dried fruit. Potent carotene effect was appeared at concentration of 500 μg/ml for treated lymphocytes with increasing CD3 level to get upper level after 2 hours interval, while after 4 hours exposure both CD4 and CD8 levels increased dramatically for lymphocytes treated with 125 μg/ml, and 250 μg/ml Lycium carotene. An alteration in cytokines gene expression for IL-10 production in response to carotene treatments at (125, 250, 500) μg/ml for both intervals, with suppress in tumor necrosis factor (TNF-α) gene expression in relation to β-actin the internal control and the PHA mitogenic agent. Conclusion: The study concluded that the carotenes extracted from Lycium barbarm fruits seemed to initiate the immune system toward Th2 cell activation rather than Th1, besides the extract may potentiate IL-10 production. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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