| Autor: |
Mansyah, Ellina, Sutanto, Agus, Santoso, Panca Jarot, Prihatini, Riry, Makful, Sukartini |
| Předmět: |
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| Zdroj: |
AIP Conference Proceedings; 2023, Vol. 2616 Issue 1, p1-8, 8p |
| Abstrakt: |
Mangosteen is classified as an apomictic plant due to its limited genetic diversity. The genetic differences between mangosteen can be determined by analyzing polymorphism and monomorphic DNA fragments. The purpose of this study was to identify and characterize mangosteen DNA fragments using RAPD analysis. Seven mangosteen accessions from West Sumatera, Riau, Jambi, and West Java were used in the study. DNA extraction, PCR amplification, DNA fragment separation, cloning, sequencing, and BLAST analysis (http://blast.ncbi.nlm.nih.gov/Blast.cgi) were used in this study. 69 DNA fragments were extracted and sequenced through RAPD analysis. Homology analysis revealed that 82% of the sequences contained transposons and microsatellites. Two segments homologous to calcium/calmodulin-dependent protein kinase II delta (CAMK2D) and KATAMARI1/MURUS3 were discovered in non-gamboge disease samples. This enzyme is involved in calcium ion regulation, endomembrane organization, and xyloglucan galactosyltransferase, all of which are necessary for the physiological mechanisms of the cell wall. These findings can be used to further our understanding of the mechanisms underlying genetic variation and gamboge disease in mangosteen. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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