Das FuN Screen-Prinzip zur experimentellen Analyse von Nanoporen in E. coli.

Autor: Weber, Wadim, Probanowski, Tobias, Eisenhauer, Klara, Kemp, Philipp, Stein, Viktor
Zdroj: BioSpektrum; May2023, Vol. 29 Issue 3, p315-317, 3p
Abstrakt: Nanopores constitute an important class of biotechnologically relevant proteins. Unlike binders and enzymes, their experimental characterization is limited to high-resolution, yet low throughput biophysical methods. Addressing this technological gap, the functional nanopore (FuN) screen now provides a versatile assay to study and engineer nanopores in Escherichia coli combining quantitative resolution with the ease, scalability, and throughput of cellular assays. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index