Chemical profiling and cytotoxic potential of the n-butanol fraction of Tamarix nilotica flowers.
| Autor: | Fayed, Marwa A. A., Bakr, Riham O., Yosri, Nermeen, Khalifa, Shaden A. M., El-Seedi, Hesham R., Hamdan, Dalia I., Refaey, Mohamed S. |
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| Předmět: |
ADENOCARCINOMA
IN vitro studies STATISTICS MEDICINAL plants LIVER tumors PHENOLS FLAVONOIDS LIQUID chromatography ONE-way analysis of variance ANTINEOPLASTIC agents TANNINS NUCLEAR magnetic resonance spectroscopy PHYTOCHEMICALS PLANTS CELLULAR signal transduction CELL survival COMPARATIVE studies FLOWERS MASS spectrometry DESCRIPTIVE statistics DOSE-effect relationship in pharmacology PLANT extracts CELL lines MOLECULAR structure ALCOHOLS (Chemical class) DATA analysis DATA analysis software BREAST tumors METABOLITES PHARMACODYNAMICS |
| Zdroj: | BMC Complementary Medicine & Therapies; 5/24/2023, Vol. 23 Issue 1, p1-14, 14p, 1 Color Photograph, 2 Diagrams, 4 Charts, 4 Graphs |
| Abstrakt: | Background: Cancer represents one of the biggest healthcare issues confronting humans and one of the big challenges for scientists in trials to dig into our nature for new remedies or to develop old ones with fewer side effects. Halophytes are widely distributed worldwide in areas of harsh conditions in dunes, and inland deserts, where, to cope with those conditions they synthesize important secondary metabolites highly valued in the medical field. Several Tamarix species are halophytic including T.nilotica which is native to Egypt, with a long history in its tradition, found in its papyri and in folk medicine to treat various ailments. Methods: LC–LTQ–MS–MS analysis and 1H-NMR were used to identify the main phytoconstituents in the n- butanol fraction of T.nilotica flowers. The extract was tested in vitro for its cytotoxic effect against breast (MCF-7) and liver cell carcinoma (Huh-7) using SRB assay. Results: T.nilotica n-butanol fraction of the flowers was found to be rich in phenolic content, where, LC–LTQ–MS–MS allowed the tentative identification of thirty-nine metabolites, based on the exact mass, the observed spectra fragmentation patterns, and the literature data, varying between tannins, phenolic acids, and flavonoids. 1H-NMR confirmed the classes tentatively identified. The in-vitro evaluation of the n-butanol fraction showed lower activity on MCF-7 cell lines with IC |
| Databáze: | Complementary Index |
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