| Abstrakt: |
Background and Objectives One of the antigens of the blood group is Duffy, which includes different subtypes. This system is of clinical importance in terms of blood transfusion, so accurate identification of this antigen is very important. In this study, the process of solubilization of the membrane of red blood cell (RBC) and purification of the Duffy antigen, were investigated. Materials and Methods In this experimental study, packed RBCs (O- and Fya+) were obtained from the blood donation center of Tehran province. The membranes of RBCs were solubilized using Nonidet P-40 detergent. In the next step, Fya antigen was purified by affinity chromatography method using a specific antibody against this antigen. The specificity of the purified protein was tested by ELISA technique Results The Fya+ characteristic of red blood cells of the RBC bag was confirmed. In addition, the specificity of the antigen was confirmed by ELISA method. The absorbance levels of the purified antigen (OD450nm) in ELISA method were 1.056 ± 0.137 and 0.197 ± 0.016 by employing the Fya and Fyb antibodies, respectively. Conclusions The present research showed that red blood cell membrane solubilization can be done to purify Fy antigen and this antigen may be used in various fields including immunization and antibody production. [ABSTRACT FROM AUTHOR] |
