| Abstrakt: |
Glycine max is one of the important oilseed plants in the world. One of the problems of G. max production is seed deterioration and low germination and vigor during seed storage and before planting time. To investigate the effects of deterioration on seed and the effects of salicylic acid and ethylene on the improvement of the deteriorated seed of G. max, an experiment was conducted accelerated aging test for 0, 6 and 10 days and a natural aging test for 6 months. After aging conditions, seeds were imbibed with 50 μ M salicylic acid and 10 μ M ACC (precursor of ethylene) for 6 hours at 25 °C. Also, some seed was used without any hormonal treatment as a control seed (called dry seed) after the natural and accelerated aging test. Germination percentage, the activity of catalase, superoxide dismutase and glutathione peroxidase enzyme, total protein, and malondialdehyde were measured. CTR1 and NPR1 Gene expressions were investigated on dry seed and under imbibitions of water, salicylic acid and ACC at 6, 12 hours with the Q-RT-PCR method. Seed germination decreased; malondialdehyde content increased and total protein decreased. Catalase, superoxide dismutase and glutathione peroxidase enzyme activities were decreased and electrical conductivity increased with the progress of aging. Gene expression varied at different days and different hours. Salicylic acid and ACC had different effects on measured traits. Totally, aging caused seed physiology disorder and salicylic acid and ACC were not able to improve deteriorated soybean seed. [ABSTRACT FROM AUTHOR] |
