Biochemical Response of Human Endothelial and Fibroblast Cells to Silver Nanoparticles.

Autor: Vivas, Cristofher Victor, dos Santos, Jennifer Adriane, Barreto, Yan Borges, Toma, Sergio Hiroshi, dos Santos, Jonnatan Julival, Stephano, Marco Antonio, de Oliveira, Cristiano Luis Pinto, Araki, Koiti, Alencar, Adriano Mesquita, Bloise, Antonio Carlos
Zdroj: BioNanoScience; Jun2023, Vol. 13 Issue 2, p502-520, 19p
Abstrakt: Bactericidal and antimicrobial properties of silver nanoparticles, AgNPs, in addition to their cytotoxic effects, have been investigated to properly modulate cell biochemistry processes in order to improve the healing of wounds. Herein, we studied the cytotoxicity and metabolic profiling of two human cell lineages, fibroblast FN1 and endothelial HUV-EC-C, planning doses of AgNPs, and incubation times. Cytotoxicity assays showed consistent decrease in proliferation rates, viable cell number, and average surface areas. Metabolomics and nuclear magnetic resonance were successfully used to characterize changes in metabolic events triggered by silver treatments. Endo- and exometabolome revealed biochemical changes induced on treated cells compared to controls. For instance, glycolytic pathway is up-regulated due to the elevation in glucose consumption; however, the consequent elevation in pyruvate production seems to be wasted by cells to generate energy by aerobic means that are choosing to oxidize it to acetate. Aminoacid metabolism is down-regulated, signalizing the protein degradation mechanism. Tricarboxylic acid cycle is down-regulated once succinate was left over in the culture media. Concurrently, the ketogenic pathway is up-regulated due to the excess of acetone. Changes in pyroglutamate metabolism were detected indicating up-regulation of glutathione biosynthesis used to equilibrate the effects induced by oxidative stress, in accordance with the N-acetylcysteine finds. Phospholipid metabolism is down-regulated, as revealed by the changes in O-phosphocholine and Sn-glycerol-3-PC levels, signaling reduction in the cellular proliferation rates. To the best of our knowledge, this is the first report describing AgNP-induced changes in the UDP-GlcNAc levels, which plays an essential role in modifying nucleocytoplasmic proteins. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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